Projects

2009
Colletotrichum truncatum is a pathogen of several leguminous plant species where it causes a disease called anthracnose. Host specialization, infertility and genetic differentiation among isolates from different hosts have been demonstrated. In the population of C. truncatum from lentil two races were described. Detailed histological studies of the infection process by isolates of both races have revealed quantitative rather than qualitative differences.
2009
Lentil anthracnose is currently the most important lentil disease in Saskatchewan. In a project about to be completed on molecular aspects of this pathogen, we generated an extensive library of genes activated by the anthracnose fungus and by lentil during the infection process. In order to fully benefit from the investment made in this NSERC-CRD, we received one-year funding to confirm the function of a number of genes in the anthracnose fungus, Colletotrichum truncatum, that we have identified and believe to be involved in virulence of the fungus.
2009
The project has three phases: In the first phase, chickpea genotypes were evaluated in the growth chambers for their flowering response under both long (16 h) and short days (10 h) and 22 0C and 16 0C day and night temperatures. Variability among the genotypes in their flowering response under either long or short days was identified. In the second phase of the study eight selected chickpea genotypes with extreme responses to photoperiod will be evaluated to determine the timing and duration of the photoperiod sensitive phase and the time of floral initiation and to establish whether photoperiod sensitivity ends at floral initiation or if it extends further into the phases of flower development. These same eight genotypes will be further characterized in a factorial combination of two photoperiods: 10 h and 16 h and three temperatures regimes: 16/8 0C, 20/12 0C and 24/16 0C (day/night). This study allows us to determine flowering response of chickpea genotypes grown in a range of thermal regimes combined with either long or short days. In the third phase of the study, chickpea RILs derived from a cross between ICCV 96029 and CDC Frontier and their parents will be used for mapping genes for early flowering, photoperiod insensitivity and reaction to ascochyta blight.
2009
Approximately 60-80% of total phosphorus is stored in crop seeds as phytate. Phytate is not readily available to humans and non-ruminant livestock because of their lack of phytase enzyme. The low-phytate lines had similar seedling emergence counts, vine length, lodging score, and mycosphaerella blight score when compared with CDC Bronco. The low-phytate lines had somewhat later days to flowering and days to maturity, and somewhat lower grain yield and seed weight than CDC Bronco. Harvested seeds of the low-phytate lines had substantially higher inorganic phosphorus (1.21-1.28 mg/g) concentration than CDC Bronco (0.24-0.25 mg/g) and the other normal-phytate cultivars.
2009
Double haploids are plants developed from either a male or female gamete, n=1 cell, and therefore are completely homozygous at all loci. Because all traits are visible within one generation, this methodology adds speed and efficiency to breeding programs. The goal of our research is to improve all aspects of the field pea anther culture protocol including: increasing the number of immature pollen grains initiated to become embryogenic, improving the regeneration of haploid embryos, and regenerating plants from those embryos.
2009
A set of 1107 legume cross species orthologous sequences (COS) were amplified from Lens culinaris (CDC Redberry and Eston) and L. ervoides (L01-827a and IG 72815). Sequences were aligned and SNPs identified. A subset of 110 KASP assays were designed for use in L. culinaris. An Illumina GoldenGate array of 768 SNPs was designed for use in L. ervoides or interspecies hybrid populations between Lc and Le.
2008 to 2009
Mixture of eight cultivars with varying seed phenotypes: Indian Head, Commando, CDC LeMay, CDC Robin, and breeding lines 1899T-50 and 1788-4 (CDC, Univ. Saskatchewan, Saskatoon, Canada) All developmental stages of seeds and very young fertilized pods were harvested from mature plants, and divided into the following lots: very young fertilized ovaries, young ovules, enlarging seeds, cotyledons of fully filled seed, seed coats of fully filled seeds. cDNA library was made from a mixture of equal amounts of mRNA extracted from each of the above tissues.
2009
<p>Preparation of EST data: Sequences were extracted from dbEST and were subjected to quality control screening (vector, E. coli, polyA, T, or CT removal, minimum length = 100 bp, &lt; 3% N). Preparation of transcript (ET) database: All sequences from the appropriate divisions of GenBank (including RefSeq) were extracted. Non-coding sequences were discarded and cDNAs and coding sequences from genomic entries were saved. Sequences and related information (e.g. PubMed links) are stored in the qcGene database (qcGene). Assembly: Cleaned EST sequences and non-redundant transcript (ET) sequences were combined. Using the Paracel Transcript Assembler Program, sequences were assembled into contigs. TCs are consensus sequences based on two or more ESTs (and possibly an ET) that overlap for at least 40 bases with at least 94% sequence identity. These strict criteria help minimize the creation of chimeric contigs. These contigs are assigned a TC (Tentative Consensus) number. TCs may comprise ESTs derived from different tissues. The best hits for TC's were assigned by searching the TC set against a non-redundant amino acid database(nraa) using BLAT. The top five hits based on score were selected and displayed for each TC. Caveats: TCs are only as good as the ESTs underlying them; there may be unspliced or chimeric ESTs and thus TCs. There is still redundancy in the TC set because sequences must match end to end and at a certain percent identity to be combined. Directionality of the TCs should not be assumed. Not all TCs contain protein-coding regions.</p>
2009
In many important crop species, the strategy of single seed descent (SSD) enables only 2 - 3 generations per year. Approximately eight generations of inbreeding are required before plants are mostly homozygous (‘true breeding’). This creates a ‘bottleneck’ in cultivar development. Hence, the purpose of this project is to develop a rapid generation cycling technique for CDC pulse crops in order to speed up the breeding process by using in vitro flowering technique.
2009
Lentil has been grown commercially in western Canada since 1970. Ascochyta lentis, the causal agent of ascochyta blight of lentil is established as one of the most economically important diseases of lentil in Western Canada. To deal with this problem, the widely acceptable genetic improvement strategy is to pyramid resistance genes. Developing closely linked single nucleotide polymorphism (SNP) markers for resistance genes is prerequisite for pyramiding resistance genes. To develop SNP markers, a series of selected recombinant inbred line (RIL) populations derived from resistant sources will be phenotyped under greenhouse conditions (pathogenicity tests) followed by screening available SNP markers across the entire set of RIL populations.

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