SNP genotyping of genes governing resistance to Ascochyta lentis in cultivated and wild lentil

Overview
2009

Lentil has been grown commercially in western Canada since 1970. Ascochyta lentis, the causal agent of ascochyta blight of lentil is established as one of the most economically important diseases of lentil in Western Canada. Resistance breeding is important for developing a durable and sustainable strategy for managing problems with ascochyta blight. Germplasm sources with genes for resistance to ascochyta blight have been identified both in the cultivated and the wild Lens species.  However, large scale and long-term cultivation of lentil cultivars with single resistance genes may enable the pathogen to overcome the resistance. To deal with this problem, the widely acceptable genetic improvement strategy is to pyramid resistance genes. Developing closely linked single nucleotide polymorphism (SNP) markers for resistance genes is prerequisite for pyramiding resistance genes. To develop SNP markers, a series of selected recombinant inbred line (RIL) populations derived from resistant sources will be phenotyped under greenhouse conditions (pathogenicity tests) followed by screening available SNP markers across the entire set of RIL populations. To improve phenotyping and to understand the potential variation in physiological reactions of different resistance genes, the histological reaction to infection with the pathogen will also be examined using microscopy facilitated by using at fungal isolates genetically transformed with a green fluorescent protein (GFP) reporter gene.  The results of this research will be fundamental to developing a sustainable genetic improvement strategy for lentil based on developing lines which carry multiple resistance genes for use in creating durable resistance to ascochyta blight.

Properties
Additional information about this project:
Property NameValue
TypeResearch Experiment
Research Area
Breeding & Genetics

Plant breeding is the art and science of changing the traits of plants in order to produce desired characteristics. Plant breeding can be accomplished through many different techniques ranging from simply selecting plants with desirable characteristics for propagation, to more complex molecular techniques. ... [more]

 
Pathology

In the Pulse Crop Pathology Group we are interested in the biology of fungal and bacterial pathogens and their interaction with the legume host plants. The ultimate goal is to gain a better understanding of strategies employed by these pathogens to successfully invade and colonize pulse crops, and to explo ... [more]

 
Related Species

Lentil (Lens culinaris Medik.) is an important pulse crop with annual production of 3-4 Mt across 70 countries (Cubero et al. 2009. DOI 10.1079/9781845934873.0000; pg. 13). Lentils are a good source of protein, carbohydrates, micronutrients and vitamins for human nutrition and is consumed in more than 120 countries. Furthermore, their small seed size and flat shape make them relatively quick cooking and easily decorticated compared to most other grain legumes (Sharpe et al. 2013. BMC Genomics. DOI 10.1186/1471-2164-14-192). The Lentil plant has a bushy growth habit with a height of about 40 cm; the seeds are lens-shaped and usually grow two per pod. ... [more]

 

Lens ervoides is a wild relative of Lens culinaris with semi-hastate or lanceolate stipules. L. ervoides can be distinguished from other wild Lentil species by its smaller leaves, calyx teath, pods and seeds1. L. ervoides is often found in shady or partially shady niches, such as among bushes or under trees, with stony soils1. Unlike other wild lentil species, L. ervoides is rarely found in mixed stands with other wild lentils1. Recent sequence analysis indicates that Lens ervoides is in the tertiary gene pool of L. culinaris2 ... [more]

 

Lens lamottei is a wild relative of Lens culinaris with horizontal, less dentate stipules1. Recent sequence analysis indicates that Lens lamottei is in the secondary gene pool of L. culinaris2. ... [more]

 

Lens nigricans is a wild relative of Lens culinaris. L. nigricans has stipules that are considerably semi-hastate and dentate at their base1. This species can be divided into two groups based on the orientation of their stipules. Those accessions with upright stipules are usually found in gravelly soil in southern Spain, southern Italy and along the Adriatic coast of Yugoslavia, as well as in man-made habitats throughout southern Europe; whereas, those accessions with horizontal stipules were found on calcereous or basaltic soil in stony and gravelly habitats in Israel and Turkey1. Recent sequence analysis indicates that Lens nigricans is in the quaternary gene pool of L. culinaris2 ... [more]

 

Lens odemensis is a wild relative of Lens culinaris with semi-hastate stipules which form horizontal positions on the stem1. Recent sequence analysis indicates that Lens odemensis is in the secondary gene pool of L. culinaris2. ... [more]

 

Lens orientalis is a wild relative of Lens culinaris with lanceolate stipules. The geographical distribution of Lens orientalis ranges from Turkey to Uzbekistan with a primary habitat of stony and gravelly niches where aggressive annuals are not successful1. Lens orientalis usually forms small disjunct populations containing a small number of plants in sparse stands1. More extensive populations of L. orientalis were found at high elevations (800 to 2,000 m)1. Recent sequence analysis indicates that Lens orientalis is in the primary gene pool of L. culinaris2 ... [more]

 

Lens tomentosus is a wild relative of Lens culinaris. Morphologically, L. tomentosus most resembles L. orientalis although it can be distinguished as having a hairy pod2. Recent sequence analysis indicates that Lens orientalis is in the primary gene pool of L. culinaris2. ... [more]

 
Sequences, Variants & Markers